Enabling Purification Technologies - Ultra-high Resolution
Conventional batch chromatography suffers from three drawbacks:
- there is a big discrepancy between the selectivity of analytical separations and preparative separations making it difficult to correlate analytical column chromatography profiles with preparative column chromatography profiles .
- the preparative batch chromatography profile, being quite coarse, has to be fractionated and pooled fractions have to be further processed using an orthogonal separation matrix to obtain a reasonably pure product.
- When scaling up for high throughput, batch chromatography created large back pressures that limit its scale and resolution
The ContiChrom platform can by-pass these disadvantages by employing the following principles that act synergistically to provide high resolution leading to high product yield and purity with superior throughput.
- As several columns are used in continuous chromatography, the individual column dimensions are smaller, allowing using small particle matrices with high resolution power.
- The lower back-pressure of the system allows to perform the run at higher pressures.
- The internal re-cycling mode provides an enhanced engineering solution to a separation problem resulting in higher yield and purity.
- The counter-current gradient elution mode enhances the resolution further.
In the figures shown below the difference between a standard preparative batch chromatography profile and a ContiChrom profile are presented.
